Technologies

Technologies

List of Technologies present on the RHEM platform

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Hybridation In Situ par la Technologie RNAscope

RHEM_AdV provides access to fully equipped workstations featuring RNAscope® technology, offers custom services and supports user in developing customised protocols tailored to their specific research needs.

RNAscope® technology enables the visualization, localization and spatially quantification of target RNA or microRNA expression at the cellular or tissue level. Detection can be performed using chromogenic or fluorescent staining, depending on the desired readout.

This technology can also be combined with immunofluorescence, allowing simultaneous visualization of RNA and protein expression within the same sample.

RNAscope

Advantages of this technology:

  • High specificity and sensitivity, thanks to a double ‘Z’ probe design that minimize non-specific signals and enables strong signal amplification,
  • Targeting of short regions (40-50 bases), enabling the detection of partially degraded RNAs,
  • Multiplexing capability, allowing the detection of up to 3 simultaneous targets, together with nuclear conterstaining (DAPI),
  • Compatibility with various tissue preparation, including microtomy, cryotomy, vibratomy.

 

Specific approaches available on the platform:

  • RNAscope® Multiplex Fluorescent V2 with or without protease
  • miRNAscope
  • RNAscopePlus smRNA_RNA
  • RNAscope duplex