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Technologies
List of Technologies present on the RHEM platform
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Sectioning methods
Several sectioning methods are offered by RHEM to meet the needs of research projects requiring histological analysis of samples. The different sectioning methods are illustrated and explained below.
RHEM can provide guidance on selecting the appropriate method based on the biological question.
Serial sections on one or multiple slides (S): the organ is reconstructed on one or more slides.
Serial/serial sections reconstructing the organ across multiple slides (S/S): the organ is reconstructed on one or more slides. Slides 1, 2, and 3 are mirrored to each other within a few micrometers.
Dotted sections on one or multiple slides (S): indicate that multiple sections of the organ are present on the same or several slides, with several micrometers separating each section.
Serial/dotted sections reconstructing the organ across multiple slides (S/P): the organ is reconstructed over several slides. Slides 1, 2, and 3 are mirrored to each other. Three series of serial sections are made (1/2/3, 4/5/6, 7/8/9) with several micrometers separating sections 3 and 4, 6 and 7, and so on.
Multi-block/serial sections across multiple slides (MB/S): indicate that the organs from each block are reconstructed over several slides, with one section from each block present on each slide. Slides 1, 2, and 3 are mirrored to each other within a few micrometers.
Multi-block/dotted sections across multiple slides (MB/P): indicate that one section from the organs of each block is present on the same slide, with several micrometers separating sections from each block. Slides 1, 2, and 3 are mirrored to each other, but several micrometers separate sections 1 and 3, 3 and 5, and so on.
