Equipment
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List of Equipment present on the RHEM platform
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Cryostat Microm HM550-OMPVD
Description:
Cryosectioning of samples from 5 µm to 30 µm can be performed using fresh or fixed material. All types of pre-prepared samples can be sectioned with a cryostat.
The cryostat is a cryogenic device that maintains a low temperature ranging from +10 °C to –50 °C within a thermally insulated chamber. A microtome housed inside this chamber enables the cutting of tissues hardened by freezing (from 4 µm to 30 µm). The system is also equipped with a section de-wrinkling device using suction and a disinfection system that vaporises a disinfectant solution. Cryostats can produce serial or non-serial sections from fixed or unfixed tissues. Sections are then transferred onto specialised histological slides (such as Superfrost +, Probe On gelatin-coated, or silanised slides).
These high-performance instruments are used for routine work and research in biology and medicine, as well as in industrial applications.
Applications in optical microscopy are diverse, ranging from simple structural staining to immunolabelling with a wide range of markers: multiple fluorochrome labelling, double enzyme labelling, or double labelling with colloidal gold and silver. Cryosectioning is also a method of choice for in situ hybridisation.

Cryostat Microm HM550-OMPVD


